Examine the QTL understood within this analysis that have in past times authored P. striiformis f. tritici opposition family genes and you may QTL, and to get physical ranks, the resource succession and you will marker alignments from polymorphic SNP markers and you can the brand new SNP probes was in fact aligned for the Chinese Spring series due to a-blast research (IWGSC RefSeq v1.0; CS genome hereafter). New SNP probes was together with blasted from the Svevo genome sequences at GrainGenes Svevo Genome Browser (Durum Wheat Svevo RefSeq Rel. step 1.0; durum genome hereafter).
Seedling tests with plants of susceptible and resistant RILs and of Svevo inoculated at different stages with the Israeli race (38E134) showed that plants were susceptible before the four-leaf stage but plants at the five-leaf stage began to show stripe rust resistance. Svevo at the five-leaf stage had IT 3 to 4, which was much lower than the IT of the most resistant RIL (Fig. 1A). To determine the broadness of the resistance in Svevo, six P. striiformis f. tritici races were used to inoculate Svevo together with susceptible control cultivars Zavitan, Falchetto, and MX169. The results showed that Svevo was susceptible (IT 8 to 9) to all six races (one from Israel and five from China) in the seedling tests but highly resistant (IT 1) in the adult-plant tests to all races. The susceptible parent, Zavitan, and MX169 and Falchetto were susceptible (IT 8 to 9) at both growth stages. Svevo had consistent IT 1 in the adult-plant stage under field conditions, whereas Zavitan, MX169, and Falchetto displayed IT 9 in all environments with a severity of 90 to 100% (Fig. 1B). The IT and DS values of the 137 RILs were in the range of 0 to 9 and 0 to 100%, respectively, indicating that resistance was quantitatively inherited (Fig. 2). In general, the ITs in Israel were higher for most of the lines than the ITs in China (Fig. 3). The broad-sense heritability of IT and DS was 0.91 and 0.92, respectively. Correlation coefficients (r) between IT and DS varied greatly between the 11 environments (r = 0.36 to 0.98) (Supplementary Table S1). The correlation coefficients were higher between the environments in China or in Israel but lower between environments in the two countries. In the pairwise comparison of the IT or DS, the values of r were all significant (P < 0.001) (Table 1), although the values of r between years were slightly lower than that within years. The ANOVA results based on both the IT and DS data showed that the interactions among the RILs, environments, and line–environment were significant (P < 0.01) (Table 2). No significant variation was detected among replications within each experiment, and the lines were the main source of high-heritability phenotypic variation.
Fig. 1. Stripe corrosion reactions for the seedling at stages from A great, 1 to 2, two to three, and 4 or 5 leaves regarding Svevo (SV), unwilling (R), and you may prone (S) recombinant inbred contours and you can B, to your banner simply leaves out of Mingxian 169 (MX169), Svevo, and you may Zavitan.
Fig. dos. Volume distributions regarding indicate disease designs (0 so you can nine) and state severities (0 in order to 100) to have 137 recombinant inbred lines regarding mix Svevo ? Zavitan adult into the China and Israel into the 2016 in order to 2018.
Fig. step three. Stripe corrosion scores depicted of the average disease kind of (IT) and you may disease severity (DS) regarding recombinant inbred traces of Svevo ? Zavitan inside eleven environment when you look at the Israel and you can China.
Desk step one. Data off difference and you will estimate regarding wide-sense heritability of disease type of (IT) and you can situation seriousness (DS) among recombinant inbred outlines from Svevo and you can Zavitan checked in the China and you may Israel within the 2016 to help you 2018